Starch hydrolysis test: Starch agar can be used to determine in the event that bacteria generate the chemical amylase which will breaks down starch, causing hydrolysis. test, decarboxylation, deamination,Bile Esculin, Starch hydrolysis, Urea, Casein, Gelating hydrolysis, DNA hydrolysis, Lipid hydrolysis, SIM and any 4. 4. This is determined using a Gram Stain process. Starch hydrolysis test: starch agar is used to determine if bacteria produce the enzyme amylase which breaks down starch, causing hydrolysis. The culture was shaken, then immediately 10 drops of Barritt’s reagent B was added and it was shaken again. The Biochemical identification of Serratia marcescens showed that bacteria were Gram –ve, Rod, Catalase positive, oxidase negative, lactose non- fermenter, motile, ... Bacillus subtilis is positive for starch hydrolysis (pictured below on the left). Serratia marcescens is a species of rod-shaped gram-negative bacteria in the family Enterobacteriaceae. Each organism was used to inoculate a liquid nutrient broth which was then incubated at 37◦C for 18-24 hours. 2. As with many other . Provide a flow chart/dichotomous key to all tests you did for your organism. Serratia marcescens (/ s ə ˈ r eɪ ʃ i ə m ɑːr ˈ s ɛ s ɪ n z /) [failed verification] is a species of rod-shaped, Gram-negative bacteria in the family Yersiniaceae.It is a facultative anaerobe and an opportunistic pathogen. by admin March 5, 2016, 12:26 am 1.8k Views. For the methyl red test, five drops of pH indicator methyl red is added to one of the tubes of medium. Both isolates were tested for oxidase by adding p-aminodi-methlyaniline oxalate to colonies grown on a medium, and neither had a reaction, indicating that they do not produce cytochrome oxidase. After inoculation, the plate was incubated at 37◦C for 24 hours. • *starch hydrolysis test (amylase) • *PR-glucose, PR-lactose, PR-sucrose tests (fermentation) ... Serratia marcescens Shigella flexneri Gram-positive Bacillus cereus Bacillus megaterium Bacillus subtilis Enterococcus faecalis Lactococcus lactis Micrococcus luteus A human pathogen, S. marcescens is involved in hospital-acquired infections (HAIs), particularly catheter-associated bacteremia, urinary tract infections and wound infections, and is responsible for 1.4% of HAI cases in the United States. You must cite our web site as your source. After inoculation, the slant tubes were incubated at 37◦C for 18-24 hours. Some individuals are of the opinion that this imported food has detrimental effect on our economy and culture and it would be better if people ate only the local produce. Starch Hydrolysis Several drops of Gram’s iodine were added to the bacterial streaks on the starch agar plate. When the Gram stain was inconclusive for this bacterium, it was sensible to use other tests to positively identify it. The Safe Assign tool will be turned on to make sure that the work you are submitting your original work. Biochemical Test and Identification of Serratia marcescens. The pure culture of the isolate I-3 is shown in Fig-1B. ' Requirements: Media: DNase Agar or DNase agar with Methyl green indicator. Any bacteria that seemed to repel the iodine, creating a clear area around the streak, were considered to have gone through starch hydrolysis (positive outcome). The Essay on Some people think imported food exerts positive impacts on our lives. Please include the following: According to him, coercion is the intention to interfere in the freedom of an individual. Binomial name. The red growth is labeled #1 and the white growth #2. However, few people know that their cell walls are quite different. Oxidase test: This test is designed to distinguish among groups of bacteria based on cytochrome oxidase activity. Serratia marcescens is a species of rod-shaped gram-negative bacteria in the family Enterobacteriaceae. 25°C) and 3 to 14 pH (opt. (T. W. Firm, Producer) Retrieved May 10, 2009, from Serratia- Marcescens.org: http://www.serratia-marcescens.org/index.cfm Katelijne Dierick, e. a. Observation of the bacterium with oil immersion, seemed to indicate a diplococcus, however after completing the aforementioned physiological and biochemical tests, it must be concluded that isolate #1 is probably a short rod. 4. Once it is determined to be a gram-negative rod, the oxidase test being negative leads to doing a methyl red test. Figure: DNA hydrolysis. Baltimore: The Williams & Wikins Company. The methyl red test is used to identify enteric bacteria based on glucose metabolism. IS serratia marcescens negative for a starch hydrolysis test? environmental species). Red and blue wavelengths of light are ... ... phosphate levels affect autotrophic organism? MQCL 49, E85/66 E85/66; MQCL 49 Serratia marcescens SNELL J J S CPHL COLINDALE The National Collection of Type Cultures comprises over 5000 bacterial cultures, over 100 mycoplasmas and more than 500 plasmids, host strains, bacteriophages and transposons. It does not contain the ideas, or writing of other individuals/authors. 5. In liquid media, the cells are short rods known as swimmers, … Because of this, the illness often goes unreported. (boydii, dysenteriae, flexneri) Shigella sonnei … In this case, microbes Bacillus subtilisin and Escherichia coli are tested. Looks like coccobacilli. Biology Educational Videos Table of Contents hide Biochemical Test of Serratia marcescens Fermentation of Enzymatic Reactions Biochemical Test of Serratia marcescens Basic Characteristics Properties (Serratia marcescens) Capsule Negative (-ve) Catalase Positive (+ve) Citrate Positive (+ve) Flagella Positive (+ve) Gas Variable Gelatin Hydrolysis Positive (+ve) Gram Staining … The plate was then flooded with Gram’s iodine which reacts with starch to turn purple-blue. Pseudomonas aeruginosa and Serratia marcescens with clear transparent zones while most other organisms failed to produceanyhydrolysis orat the most, hazy zones only. Table-3: Biochemical tests for the selected isolate I-3 S.No Biochemical Tests Isolate I-3 1 Chitin hydrolysis + 2 Starch hydrolysis + 3 Gelatin hydrolysis … RESULTS Based on the colony morphology, physical and biochemical test, Serratia sp is a gram negative, rod shaped bacteria and grows under room temperature. The plate was incubated at 37◦C for 24 hours. (Find a price that suits your requirements), The Essay on Biology and Population Growth Rate, The Term Paper on Identifying Unknown Bacteria, The Essay on Gram Positive And Gram Negative Bacteria, The Essay on Positive And Negative Effects Of Slavery In Cultural And Social Terms For The Typical Southerner, The Essay on Negative Liberty Positive Freedom Coercion. Gram Positive and Gram Negative Bacteria It is a well-known fact that bacterial cells, like plant cells, are surrounded by a cell wall. Observation shows there is a bright red growth but no change in the appearance of the medium. Author: Mark Cooper Jr. It was discovered in 1819 by Bartolomeo Bizio in Padua, Italy. Positive and Negative Effects of Slavery in Cultural and Social Terms for the Typical Southerner The slavery in the U.S. was abolished more than a century and a half year ago. Serratia marcescens (/ s ə ˈ r eɪ ʃ i ə m ɑːr ˈ s ɛ s ɪ n z /) [failed verification] is a species of rod-shaped, Gram-negative bacteria in the family Yersiniaceae.It is a facultative anaerobe and an opportunistic pathogen. Starch hydrolysis test: starch agar is used to determine if bacteria produce the enzyme amylase which breaks down starch, causing hydrolysis. Description of symptoms and signs of the patient General Background. This emetic food poisoning is common year round and can affect everyone. It is necessary to first separate the two organisms by inoculating a nutrient agar plate using the streak-plate method. The MR-VP broth has changed to a yellow-orange color. The Voges-Proskauer test is used to determine if an enteric bacterium produces acidic or neutral end products. The results of the biochemical tests confirmed that the isolate I-3 was that of Serratia marcescens . Each isolate was inoculated into a TSI slant by using a sterile needle with a stab-and-streak technique. Slavery itself is referred to as a social institution defined by law and custom as the most absolute involuntary form of human servitude. Elsevier. Mannitol, EMB, MacConkey, Blood Agar, OF-Glucose, Phenol Red Carbohydrate fermentation, MR-VP, Nitrogen reduction, Catalase, Oxidase, Citrate morphology, With the color contrast, the separation of organisms is certain and pure cultures can be grown. 4. IS serratia marcescens negative for a starch hydrolysis test? This indicates the organism is a motile, anaerobe that ferments glucose with no gas. The biochemical tests show positive to the citrate utilisation, voges prokauer, gelatine hydrolysis, cytochrome oxidase, catalase test and oxidation and It is only known that the two organisms are from vomit; one is gram-positive and the other is gram-negative. Blood agar: Blood agar can be used to cultivate fastidious organisms and also shows the hemolytic properties of some microbes. Bacillus cereus can be characterized by nausea and vomiting within ½ – 6 hours after ingesting the contaminated food. carried out from serratia bacterial sp. The starch agar plate was inoculated with dampens #1 and #2 by using a sterile trap. Here you can order a professional work. Microscopic organisms (bacteria) that hydrolysis starch outputs ∝ - amylase and oligo-1, 6-glucosidase enzymes. After inoculation, the plate was incubated at 37◦C for 18-24 hours. [23] Serratia was later renamed Monas prodigiosus and Bacillus prodigiosus before Bizio's original name was restored in the 1920s.[22]. He states: 'To coerce a man is to deprive him of freedom' (121). Differential/ Selective media, Indicates this is a gram-negative bacterium, Indicates that pyruvic acid was metabolized to neutral end products Voges- Proskauer, Red color on slant, yellow in bottom, movement away from stab site Motile, Indicates presence of catalase; facultative anaerobe, Table 2: Physiological & Biochemical Test Results – #2 Bacillus cereus Test. The MacConkey agar indicates there is no lactose fermentation, leading to the final conclusion that this organism is Serratia marcescens. Reagent: Hydrochloric acid (1mol/L) only when DNase agar without an indicator is used Others: Inoculating loop, Bunsen burner Procedure of DNase (DNA hydrolysis test) Dry the surface of agar plates before use.Each plate may be divided into sections by drawing lines on the bottom of the plate. Serratia marcescens also had a high protease yield in basal medium amended with rice bran. They also are able to grow in foods from low redox potential (Eh) of −200 mV (meat) to high redox potential of +300 mV (fruit juice). serratia marcescens, It is associated with urinary and respiratory infections, endocarditis, osteomyelitis, septicemia, wound infections, eye infections, and meningitis. Further testing is needed. It is commonly found in the respiratory and … Starch Hydrolysis (amylase) + VP (add creatine as catalyst w/ reagents) Cell Diamenter ! Without indicator. (2009). Microbiology: A Laboratory Manual (Eighth ed.). The patient presenting with vomiting likely has food poisoning due to the contamination of a starchy dish such as pasta salad or rice. Specific Question: How does phosphate levels influence population growth rate of Chlamydomonas ... nonliving factor that affects living organism. For microorganisms to utilize starch, it should first hydrolysis it since it is too huge to go through the cell layer. Retrieved May 10, 2009, from Pub Med Central: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1233987 Nutrition, C. f. (2009, April 9). Observation showed #1 was red on the slant but yellow in the butt of the tube. Nowadays, supermarkets are stocked with food products from around the world. Serratia marcescens is an opportunistic gram-negative pathogen and one of the main Enterobacteriaceae responsible for hospital-acquired infections. Bizio named the genus Serratia in honor of and Italian physicist named Serratia, and chose marcescens for the species name after the Latin word for decay . How long will the footprints on the moon last? Both organisms were inoculated onto opposite sides of the PEA plate and incubated at 37◦C for 18-24 hours. A spore stain was not done, however positive result for the starch hydrolysis test leads to the final conclusion that this unknown is Bacillus cereus. Slightly inconclusive. This is a negative reaction and indicates that pyruvic acid was metabolized to neutral end products. S. marcescens. Basically he defines negative liberty as the absence of coercion. This is not an example of the work written by professional academic writers. This indicates lysis of red blood cells occurred, which is beta-hemolysis. To what extent do you agree or disagree? In animals, kidneys use ATP for ... in the thylakoid of the chloroplast. Both bacteria had growth on the starch, however, only isolate #2 had a ring around the colonies. serratia marcescens. The identification of bacteria #1 was very easy since there are few organisms which have a red growth, and Serratia marcescens is the only one that is on our unknowns list. Cover Sheet I certify that the writing in this assignment is my individual work and is my sole intellectual property. #1 was negative for starch hydrolysis, not producing amylase. The purpose of this study is to differentiate and identify two unknown organisms provided by the instructor in a nutrient broth. Discussion/conclusion with the antimicrobial method (chemical/physical) treatment for the infection/disease or mitigation (if you have an Triple Sugar-Iron (TSI) agar test: This test was used for both isolates, #1 and #2, and is designed to differentiate based on differences in carbohydrate fermentation patterns and hydrogen sulfide production. Once you have added the iodine, make sure you do not tip the plate too much! Bergey’s Manual of Determinative Bacteriology (Seventh ed.). This test is also negative. Medium turned yellow, small amount of liquid, motile, Positive for lactose/sucrose/ glucose fermentation, Fermentation occurred creating an acidic pH in the medium. This is another contradiction of expected results and is contradicted in the next test. Provide a flow chart/dichotomous key to all tests you did for your organism. Each result was consistent with expected results for B. cereus. Here you can order a professional work. Serratia marcescens is a motile,short rod-shaped, Gram-negative, facultative anaerobe bacterium, classified as an opportunistic pathogen. 5. structural staining, Gram Stain: The organisms must first be identified as gram-negative or gram-positive. After blotting carefully, the slide is placed on a microscope and focused on the organism at the lowest magnification, progressing to the highest magnification with oil immersion. Wecon-cludefromthisstudythatthehydrolysisofcaseinis a valuable differential aid in the identification of Serratia marcescens. The starch agar plate was inoculated with isolates #1 and #2 using a sterile loop. Optimum conditions for glutaminase production by Serratia marcescens N1 in glutamine broth ... S.marcescens was cultured in production medium supplemented with starch as a sole source for ... content in food through hydrolysis of L-glutamine to L-glutamic acid and ammonia. It also differentiates lactose-fermenting bacteria from non-lactose-fermenting bacteria. It has a very quick onset and usually resolves within several hours. Serratia marcescens & Bacillus cereus Report. 3. Breed, R. S. (1957). Microbiology: Principles and Explorations (7th ed.). Bizio 1819. Negative Assumptions Youth People Positive, The Effects On Population Size And Growth In Australia, Energy transfers which take place in living organisms. other test you consider appropriate in the identification of your organism. By inoculating PEA and MacConkey agar, it was possible to conclusively identify #1 as a gram-negative organism. Gelatin hydrolysis test is helpful in identifying and differentiating species of Bacillus, Clostridium, Proteus, Pseudomonas, and Serratia. Transmission is by direct contact. Subsequent tests and procedures are described elsewhere in this report. Other symptoms may include abdominal pain and headache. Hydrolysis of starch produces simple sugars. A psychrotolerant bacterial strain of Serratia marcescens, originally isolated from a glacial site in Indian Himalayan Region (IHR), has been investigated for laccase production under different culture conditions.The bacterial strain was found to grow between 4 to 45°C (opt. A human pathogen, S. marcescens is involved in hospital-acquired infections (HAIs), particularly catheter-associated bacteremia, urinary tract infections and wound infections, and is responsible for 1.4% of HAI cases in the United States. Positive- Staphylococcus aureus; Positive- Serratia marcescens; and Negative. This indicates that the bacterium is positive for starch hydrolysis and the presence on amylase. The second organism, Serratia marcescens, might be present in the vomit due to the same contaminated food; however it could also have been in the intestines of the patient, without causing a problem. In addition, the stab pathway revealed some motility of the organism. In his essay 'Two Concepts of Liberty,' Isaiah Berlin distinguishes between two conceptions of freedom, namely negative and positive conception of freedom. The following tables show the physiological and biochemical tests performed for this study, as well as the student’s observations and interpretations. Cappuccino, J. G., & Sherman, N. (2008). Journal of General Microbiology. There being no growth and no change in appearance of the medium for either organism, this indicates there was no mannitol fermentation and they do not tolerate salt. However, it is necessary to perform the requisite tests in order to be certain that this is accurate. To differentiate organism based on their α- amylase enzyme activity In the same way, ... Chocolate agar: This medium was used to grow colonies of isolate #2 to be used in the oxidase test. Build a hypothetical clinical case of: a patient, animal, plant, or environmental problem (if applicable). enterobacteriaceae, unknown tested positive for the presence of catalase but negative for the presence of oxidase and starch hydrolysis. ... it’s an organism that can ferment lactose MacConkey media No growth for either bacteria Negative for both bacteria Phenylethyl alcohol Agar To select for gram positive Cocci PEA ... ... of society to think about the negative assumptions about youth rather than the positive ones. Image Source: Bailey and Scott’s Diagnostic Microbiology. There is also a diarrheal type of food poisoning due to B. cereus, however in this instance the illness presents only with vomiting. Certainly, the disadvantages of imported food outweigh the advantages. The effect of temperature on protease production by Serratia marcescens is shown in Fig. Serratia marcescens. 1"m (the width) Swollen Cell (containing spore) Citrate 6.5% NaCl Growth ... Serratia marcescens Serratia liquefaciens Shigella spp. It was discovered in 1819 by Bartolomeo Bizio in Padua, Italy. To do this, a few drops of 3% hydrogen peroxide were placed on a smear of each of the isolates. Most Serratia strains can grow from pH 5 to 9 and are among the bacteria that can grow in foods with water activity of 0.91–0.95. This simple sugars then pass through various mechanism to yield ATP molecules. Gram reaction, At the same time, plant cell walls are made up of cellulose, which contains no amino acids. Most people are unaware that uncooked rice is potentially a hazardous food since B. cereus can be present and actually survive cooking. A clear zone around the colonies demonstrates a positive test after the addition of 1N HCl. After inoculation, the plate was incubated at 37 C for 24 hours. This indicates the organism is a lactose/ sucrose/glucose fermenter, an anerobe and has motility. The plate was then flooded with Gram’s iodine which reacts with starch to turn purple-blue. Post author By a b; ... Starch hydrolysis, Urea, Casein, Gelating hydrolysis, DNA hydrolysis, Lipid hydrolysis, SIM and any other test you consider appropriate in the identification of your organism. After inoculation, the plate was incubated at 37¦C all day and night. Upon observing #2, the entire medium was yellow with a small amount of liquid at the bottom of the slant, and slight motility in the stab pathway. In addition, unknown showed complete resistance to the antibiotics penicillin and bacitracin. (General Background, 2009) B. cereus food poisoning is usually self limiting and not very severe. Therefore, S. marcescens is lactic acid O/F+. Oxidase enzymes play an important role in electron transport during aerobic respiration. They are gram -ve, catalase positive, oxidase negative, MR negative and VP positive bacteria. It was then observed that there was heavy growth on the plate with a clear ring around the colonies. After incubation, 2.5 ml of the medium was transferred to another tube to be used for the Voges-Proskauer test. may not apply to your organism. Serratia marcescens (S. marcescens) is a gram-negative bacillus that occurs naturally in soil and water and produces a red pigment at room temperature. As is more common specifically to . So remember, if you meet ... ... on population decline, as the Australias population growth rate remained positive. Starch is a storage polysaccride. Specimen #1 initially appeared to be gram-positive diplococci. Using the same morphological unknown organism you had on week 5 (see attached list) do the following: The starch agar plate was inoculated with isolates #1 and #2 using a sterile loop. Serratia marcescens nuclease (EC 3.1.30.2, endonuclease (Serratia marcescens), barley nuclease, plant nuclease I, nucleate endonuclease) is an enzyme. optimal growth conditions: temperatures, pH, aerotolerance Due to this, and because S. marcescens produces a reddish-orange tripyrrole pigment called prodigiosin, it may cause staining of the teeth. For the Voges-Proskauer test, 10 drops of Barritt’s reagent A is added to the second tube of medium. Serratia marcescens. Good hygiene is always important, but be aware that Bacillus cereus can be in uncooked rice, dried beans, cereals and spices, and this bacterium is resistant to heat. Table 1: Physiological & Biochemical Test Results – #1 Serratia marcescens Test, Crystal violet, iodine, alcohol, safranin. Add enough Gram’s iodine to the plate to ensure that the entire areas of growth and the agar surrounding it are covered (you should also add iodine to the negative control area). It was discovered in 1819 by Bartolomeo Bizio in Padua, Italy. The organism shown on the right is negative for starch hydrolysis. Bacterial cell walls are made up of polysaccharide chains linked to amino acids. Table 3 Population Change in Australia, ... in Australia, emphasizing the importance of studying population growth. Prevention includes refrigerating rice products right away instead of cooling before putting in the refrigerator. Organism #2 was obviously a gram-positive rod from the very first Gram stain. In this experiment we prose to test the whether variation in ... ... the population reproduces continuously (like bacteria, some insects, and domesticated rabbits) ( ... positive and presumably small (if h were 0, this would reduce to the natural model) (Growth ... assume that A >0, the negative sign in the second term ... All Papers Are For Research And Reference Purposes Only. Catalase test: This test is to determine whether the isolates can produce catalase in order to degrade toxic hydrogen peroxide. Several other tests were done, such as for detection of hemolysis and glucose fermentation. A human pathogen, S. marcescens is involved in hospital-acquired infections (HAIs), particularly catheter-associated bacteremia, urinary tract infections and wound infections, and is responsible for 1.4% of HAI cases in the United States. The initial streak-plate procedure was performed and placed in the incubator at 37◦C for 24-48 hours. First, the medium was inoculated with isolate #1, and incubated at 37◦C for 48 hours. San Francisco, CA: Pearson Benjamin Cummings. Bad Bug Book: Foodborne Pathogenic Microorganisms. It was also thought that ... they are going nowhere. Bacterium #2 was inoculated onto a blood agar plate using a sterile loop. Observation shows that for #1, there is no growth and no change in the appearance of the medium, while #2 does have growth, but with no change in the appearance of the medium. 1. hypothesis Serratia marcescens was first thought to be harmless (non-pathogenic)… This indicates no lactose fermentation, however since there is growth, this means the bacterium in gram-negative. It distinguishes the gelatinase-positive, pathogenic Staphylococcus aureus from the gelatinase-negative, non-pathogenic S. epidermidis . (Cappuccino & Sherman, 2008, p. 73) Using the fresh pure cultures grown in nutrient broth, a sample is placed on a slide and allowed to dry completely before heat setting. Disclaimer: This work has been submitted by a student. There was heavy gray growth on this enriched medium. Both bacteria had growth on … 3). Mannitol salt agar: This medium was inoculated with both specimens and incubated at 37◦C for 18-24 hours. The post Serratia marcescens first appeared on COMPLIANT PAPERS. Retrieved May 10, 2009, from United States Food and Drug Administration: http://www.foodsafety.gov/~mow/chap12.html. Using this process, the results were inconclusive for specimen #1, but #2 is definitely gram-positive rods. Black, J., & Black, L. (2008). After being gently mixed by rolling the tube between the palms of the hands, the results are observed. Serratia marcescens is a species of rod-shaped gram-negative bacteria in the family Enterobacteriaceae. Date: 10/24/12 Lab Instructor: Katherine Hovanes Lab Section # 12 Population Ecology Experiment Background: Phosphate is an abiotic factor; therefore, it is a nonliving factor that ... MacConkey agar: MacConkey agar is both a differential and selective medium used to select for gram-negative bacteria. Only #1 was used to inoculate this medium. Upon observing the growth on this plate, it is fairly obvious that one of the organisms is Serratia marcescens. It is commonly found in the respiratory and … Description of laboratory tests (experiments) specific to the suspected organism including: Observations were made at 15 minutes. Serratia marcescens. Endonucleolytic cleavage to 5'-phosphomononucleotide and 5'-phosphooligonucleotide end-products. This assignment will count as Part II of the Individual Unknown project. After inoculation, the plate was incubated at 37◦C for 18-24 hours, before the test. Methyl Red/Voges-Proskauer tests: The Methyl Red-Voges Proskauer medium was used to perform two tests. This is further indication that #2 is gram-positive, while #1 is most likely gram-negative. Hydrolyses double- or single-stranded substrate. This enzyme catalyses the following chemical reaction. The medium used is a TSI slant. Biochemical tests and special media according to whether you have a Gram +, Gram-, Acid fast organisms: Please note that you all the tests below For the next 15 minutes, the culture was shaken every 3-4 minutes. Serratia marcescens : Streptococcus faecalis : Bacillus subtilis: C. Starch Hydrolysis. Isolate #2 had no reaction so the result is, it does not produce catalase. The growth on this streak plate is bright red and white. The staining process is accomplished using first Crystal Violet, rinsing, then Gram’s Iodine for a mordant, rinsing with alcohol, then water, and finally staining with Safranin and rinsing with water. (2005, Aug). Jefferson City: John Wiley & Sons, Inc. San Francisco, et al: Daryl Fox. Comparing development trends in China with other middle-income countries. They need positive encouragement, and this can not be in negative assumptions. As expected, isolate #1 bubbled nicely, indicating the presence of catalase and allowing the conclusion that #1 is a facultative anaerobe instead of a strict anaerobe. Serratia marcescens responds to the environment with changes in shape and movement. (Find a price that suits your requirements), * Save 10% on First Order, discount promo code "096K2". Retrieved May 10, 2009, from Wikipedia: The Free Encyclopedia: http://en.wikipedia.org/ Alexander, S., & Strete, D. (2001). Wheat bran and corn starch also gave fairly good protease yield (Fig. Introduction Recently, there has been renewed interest ... ... acid anaerobic respiration in animals and bacteria is reversible whereas ethanolic anaerobic respiration ... a host of reactions in all organisms. Phenylethyl alcohol agar (PEA): PEA is a selective medium which is used to identify gram-positive bacterium. December 7, ... test, decarboxylation, deamination,Bile Esculin, Starch hydrolysis, Urea, Casein, Gelating hydrolysis, DNA hydrolysis, Lipid hydrolysis, SIM and any other test you consider appropriate in the identification of your organism. Provide a flow chart/dichotomous key to all tests you did for your organism. (Paupp) Being in slavery, people were treated as property, and were forced to serve ... As a selective medium, PEA identifies this as a gram-negative bacterium. Few aberrant results were obtained in the bacterial strains isolated. Microbiology: A Photographic Atlas for the Laboratory. Free Samples and Examples of Essays, Homeworks and any Papers, Filed Under: Term Papers Tagged With: microbiology, Science. There was no change in the medium which indicates a negative reaction and also indicates that #1 does not ferment glucose.
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